A new sensitive, specific, linear, precise and accurate RP-HPLC method was developed and validated for estimation of Sitagliptin in Bulk and Tablet dosage form. An isocratic, reversed phase HPLC method was developed. Shimadzu shim pack C18 (250mm x 4.5µm, x 5µ) column. Shimadzu Prominence-I LC-2030C plus equipped with Auto sampler as the instrument model. Mobile phase consists of mixture of Methanol: 0.01M Phosphate buffer pH 2.5 in the ratio (60:40 v/v) at a flow rate of 1.0mL /min with injection volume of 10µL. UV detection was performed at 270nm. The Linearity was established for Sitagliptin in the range of 10-60µg/ml with correlation coefficient of 0.9998. LOD and LOQ were found to be 0.197µg/ml and 0.602µg/ml respectively. Retention time of Sitagliptin were found to be 3.837mins. % Recovery was found to be 99.75-101.10 and %RSD was found with in ± 2. The method has been validated according to ICH guidelines for linearity, precision, accuracy, robustness, ruggedness, LOD and LOQ. The developed validated method was successfully applied for reliable quantification of Sitagliptin in bulk and pharmaceutical dosage form.

This research project designs a portable eight-cable robot for large-scale outdoor agriculture usage; specifically, this project designs a portable pole for the cable robot which can transport the whole system between different fields. To simplify the design, the pole will be installed onto the existing automatic moveable base, which is assuming that the pole will be installed onto the Honda's automatic ATV. Then, the system verified its stability if it can be used in the field, which means the portable poles can hold some amount of the forces with a small deflection, no tipping, and no slipping. After comparing these design parameters with other design parameters, kinematic analysis, pseudo-statics analysis, beam deflection analysis, slipping and tipping analysis, model design, and the cost estimates are done based on what is most acceptable during the project.

This paper presents a novel concept for using flying robots (e.g. quadcopters) as versatile, mobile haptic interfaces when interacting with human users immersed in virtual reality (VR). The proposed system will significantly increase the workspace of haptics-augmented VR, when compared to using fixed-base, limited workspace haptic interfaces. A quadcopter dynamics model is presented, a haptics model is presented wherein the 4-dof quadcopter is used to effect 6-dof haptic feedback (impossible to do all 6 at once), followed by preliminary experiments focusing on interaction of a quadcopter and wrench on the environment (human hand).

A new sensitive, specific, linear, precise and accurate RP-HPLC method was developed and validated for estimation of Ursodiol in Bulk and Tablet dosage form. An isocratic, RP- HPLC method was developed. Shimadzu shim pack C18 (250mm x 4.5µm, x 5µ) column. Shimadzu Prominence-I LC-2030C plus equipped with Auto sampler as the instrument model. Mobile phase consists of mixture of Methanol: Ethyl acetate: Water in the ratio (50:30:20 v/v) at a flow rate of 1.0ml /min with injection volume of 10µL. UV detection was performed at 243 nm. The Linearity was established for Ursodiol in the range of 5-30µg/ml with correlation coefficient of 0.9995. LOD and LOQ were found to be 0.011µg/ml and 0.034µg/ml respectively. Retention time of Ursodiol were found to be 2.811 mins. The % Recovery was found to be 99.20-101.69 and %RSD was found with in ± 2. The method has been validated according to ICH guidelines for linearity, precision, accuracy, robustness, ruggedness, LOD and LOQ. The developed validated method was successfully applied for reliable quantification of Ursodiol in bulk and pharmaceutical dosage form.

A new sensitive, specific, linear, precise and accurate RP-HPLC method was developed and validated for estimation of Bedaquiline in Bulk and Tablet dosage form. An isocratic, reversed phase HPLC method was developed. Shimadzu shim pack C18(250mm x 4.5µm, x 5µ) column. Shimadzu Prominence-I LC-2030C plus equipped with Auto sampler as the instrument model. Mobile phase consists of mixture of methanol and 10Mm ammonium acetate buffer in the ratio (85:15 v/v) at a flow rate of 0.5ml /min with injection volume of 20µL. UV detection was performed at 226nm. The Linearity was established for Bedaquiline in the range of 2-64µg/ml with correlation coefficient of 0.9998. LOD and LOQ were found to be 0.29477µg/ml and 0.9825µg/ml respectively. Retention time of Bedaquiline were found to be 2.9mins. The % Recovery was found to be 98.81-99.23 and %RSD was found with in ± 2. The method has been validated according to ICH guidelines for linearity, precision, accuracy, robustness, ruggedness, LOD and LOQ. The developed validated method was successfully applied for reliable quantification of Bedaquiline in bulk and pharmaceutical dosage form.